It is of note that Gabriel Spenser one on the company's actors was killed in a duel with the dramatist Ben Jonson in 1698, who many years later would be closely associated with Publication of Shakespeare's First Folio. After the Admiral's men left the Rose it was used by Worcester's men who had broken their contact with the owner of the Boar's Head at Whitechapel and lacked a venue and faced legal action. They remained at the Rose until the end of 1605, when, after becoming Queen Anne's men they moved to the recently built and licensed Red bull at Clerkenwell. The Rose is believed to have been demolished the following year.

The Rose would have been built mainly of timber, with lath and plaster used for the walls, entries in Henslowe's accounts for bricklaying imply a brick foundation several feet above the ground to support the wooden walls. The stage was painted; there was a tiring house behind the stage, for the players to 'attire' or dress in, with a storage room known as the 'hut' over it, and a flagstaff to fly a flag on performance days.

Dimensions of the Rose Theatre
Diameter approximately 72 ft
Diameter of the yard approximately 36 ft
Stage size 15 ft deep by 32 ft wide.